Device

Part:BBa_K2719007:Design

Designed by: Armando Cortés Reséndiz & María José Ugarte Orozco   Group: iGEM18_TecCEM   (2018-08-08)


Collagen V - Like Expression Device.

This part is composed by the following subparts: Two promoters, one for RFP reporter cassette and the other one for collagen (with IPTG inducible T7 promoter [BBa_R0184]). Also, it has a double terminator in both genes. A RFP and collagen V-like coding regions are on it too. This part is designed for an IPTG regulated collagen expression and it has a RFP reporter to simplify comoly selection.

"T--TecCEM--BBa_K2719007Diagram.png"

Figure 1. Diagram of BBa_K2719007, collagen V-like expression device



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1046


Design Notes

Optimized for E.coli Rossetta (DE3).


Source

Synthetic part.

References